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Tuesday, July 14, 2020 | History

2 edition of Evaluation of the algal assay procedure found in the catalog.

Evaluation of the algal assay procedure

Charles Manuel Weiss

Evaluation of the algal assay procedure

by Charles Manuel Weiss

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Published by U.S. Environmental Protection Agency, Office of Research and Development, Corvallis Environmental Research Laboratory, for sale by the National Technical Information Service in Corvallis, Or, Springfield, Va .
Written in English

    Subjects:
  • Algae -- North Carolina -- Cultures and culture media.,
  • Chlorophyll.,
  • Nitrogen.,
  • Phosphorus.

  • Edition Notes

    Statementby Charles M. Weiss.
    SeriesEcological research series ; EPA-600/3-76-064
    ContributionsUnited States. Environmental Protection Agency. Office of Research and Development., University of North Carolina (1793-1962).
    The Physical Object
    Paginationix, 58 p. :
    Number of Pages58
    ID Numbers
    Open LibraryOL17645600M

    The interlaboratory precision test: an eight-laboratory evaluation of the provisional algal assay procedure bottle test Author: Charles M Weiss ; Ronald W Helms. Evaluation of Anabaena flos-aquae as the Test Organisms in the Algal Assay:Bottle Test 78 General Discussion of Algal Assay Data 79 Nutrient Limitation Studies 79 Algal Specific Weight Coefficient 83 Effluent Study 84 Development of an Algal Assay Laboratory 85 Sampling Guidelines 85 Water Quality Parameters 86 Chemical Analyses

    A common method of fixation is to preserve the algal samples in a 4% Formalin-seawater solution (commercial 37% Formaldehyde = %) in glass jars or vials out of the light. (Note that Formalin is a hazardous chemical and should be handled and discarded properly. The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled .

    ROS production assay was performed using nitro-blue tetrazolium (NBT) test according to the method of Park et al. Briefly, μl of venous blood of healthy Sprague-Dawley (SD) rats, 50 μl of stimulants (P. acnes with and without algal extracts), positive control (polymorphonuclear leucocytes with zymosan) and negative control (culture media. Rapid Test Kit Evaluation Since the early ’s FGIS has responded to the need for fast, reliable testing for mycotoxins in grains and oilseeds. Since the program’s inception, the needs of the industry have changed and the expansion of testing to include additional mycotoxins as well as biotechnology-derived traits in grains and oilseeds.


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Evaluation of the algal assay procedure by Charles Manuel Weiss Download PDF EPUB FB2

A limnological data profile was developed for each of the bodies of water sampled. A data processing procedure was used to establish the relationship between water quality data and algal cell density, chlorophyll a. and productivity. The algal assay procedure provided an indication of limiting nutrient, phosphorus, nitrogen or both.

The interlaboratory precision test: An eight-laboratory evaluation of the provisional algal assay procedure bottle test [Charles Manuel Weiss] on *FREE* shipping on qualifying : Charles Manuel Weiss.

Get this from a library. Evaluation of the algal assay procedure. [Charles M Weiss; United States. Environmental Protection Agency. Office of Research and Development.; University of. acetone Algal Assay Procedure algal growth Anabaena Anabaena flos-aquae Appendix autoclaving bioassay biomass biomass indicators blue-green Bottle Test C0NTR0L cell counts cell production cells/ml centrifuge Chemostat chlorophyll concentration confidence interval Cyanophyceae determine distilled water dry weight elements added Eutrophication Eutrophication Research Program evaluation.

The viability of algal cells was investigated after observing the fluorescence of chlorophyll and by aid of the fluorochromes: RB – % solution in distilled water; NR – % solution in distilled water; CFW – % solution in distilled water; FDA – 2% solution in acetone.

In order to perform the CAVA test, algae were first centrifuged and re-suspended in PBS. (Figure 1). A first aliquot of re-suspended algal solution ( mL) was poured in an Eppendorf. tube and heated for 25 min at 60°C in order to completely kill the by: 3.

Request PDF | Classification and Performance Evaluation Methods of an Algal Bloom Model | A number of algal bloom models (red-tide models) have been developed and applied to simulate the redtide.

B ueltman, C. et al. - - Provisional algal assay procedure, Joint Industry/Government Task Force on Eutrophication, New York. Google Scholar F itzgerald, G. - - Detection of limiting or surplus nitrogen in algae and aquatic by: 3. Test procedures such as Practices D, D, and G7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.

The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films. In the last decades, Ecotox has widely been used for evaluation of common pollutants of aquatic ecosystems, industrial effluents, as well as the efficiency of wastewater treatment plants.

It is recommended as a fast, sensitive, and cost-effective bioassay for monitoring aquatic pollution. Linear dose-response curves for antioxidants can be generated from kinetics of the reaction.

AdVantages/DisadVantages of the TOSC Assay. The method has the advantage that it permits the quantification of the antioxidant capacity toward three oxidants, that is, hydroxyl radicals, peroxyl radicals and by: providing documented evidence that the method does what it is intended to do." Assay Validation Levels and Steps Assay Optimization (pre-validation): Assay optimization and pre-validation are experiments that determine how a range of matrix and sample elements, as well as assay conditions, effect assay parameters and assay performance.

Evaluation of algal photosynthesis inhibition activity for NDOM and EfDOM The correction of PI was conducted for each water sample by obtaining F na and the ratio of R 1p with R mp accompanied with absorbance in addition to normal algal photosynthesis inhibition test (Fig.

3 and Table S3).Cited by: 2. Algal Assay Procedure Bottle Test [National Eutrophication Research Program] on *FREE* shipping on qualifying : National Eutrophication Research Program. MTT Tetrazolium Assay Concept. The MTT (3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay was the first homogeneous cell viability assay developed for a well format that was suitable for high throughput screening ().The MTT tetrazolium assay technology has been widely adopted and remains popular in academic labs as evidenced by Cited by:   This technique, henceforth named colorimetric assay of viability for algae (CAVA), relies on the selective adsorption of erythrosine by non-viable cells and was validated on the algae species Chlamydomonas reinhardtii and Chlorella vulgaris.

The results obtained by the CAVA test were in good agreement with the in situ measurement of oxygen Cited by: 3. Algal biomass compositional analysis data form the basis of a large number of techno-economic process analysis models that are used to investigate and compare different processes in algal biofuels production.

However, the analytical methods used to generate these data are far from standardized. This work investigated the applicability of common methods for rapid chemical analysis of biomass Cited by: Each well-received μL of test solution, a nutrient spike (10 μL) and an algal inoculum (10 μL), with a final volume per well of μL (Blaise and Vasseur, ).

Peripheral wells were filled with distilled water to reduce evaporation. The test procedure involves assessment of algal growth in test chambers relative to controls by requiring a quantitative determination of algal cell numbers, and by recommending a) a qualitative appraisal of algal numbers and size by means of microscopic observation, and b) a determination of viability of growth-inhibited algae by means of mortal staining coupled with microscopic observation.

Purchase Handbook of Marine Microalgae - 1st Edition. Print Book & E-Book. ISBN. Algae convert light energy to food through photosynthesis; therefore, light may be a limiting factor in algal tests and is as essential to test performance as growth media. Although light intensity is specified under test conditions, light may be diminished or otherwise altered by the presence of color in a by: D Practices for Measurement of Chlorophyll Content of Algae in Surface Waters.

D Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water. D Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits. E Test Methods for Evaluation of Inactivators of Antimicrobial Agents.A novel method to measure algal blooms.

Posted Septem However, researchers at the USA Environmental Protection Agency recently adopted a novel method to study algae in a river system in the United States.

Algal samples are measured on filter paper with the CCM Plus Chlorophyll Meter.